Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification

Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification

Blog Article

Abstract Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming.Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria.Here, we describe a centrifugal-microfluidic chip for the detection of bacteria by integrating the cell lysis, clarification, and loop-mediated amplification (LAMP).The major advantages of this chip are as follows.Firstly, bacteria 9x11 pergola lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed.

Secondly, the on-chip assay time was short (within 70 min), which was competitive in emergency situations.Thirdly, results of the analysis can be interpreted by using a fluorescence detector or by the naked-eye, making it versatile in many areas, especially the resource-limited areas.The on-chip limits of detection of six types of bacteria were valued by gel electrophoresis, showing the similar results compared to the bench-top LAMP protocol.This chip can be used for rapid, sensitive, accurate and automated detection of bacteria, offering a promising alternative for simplifying the here molecular diagnostics of infectious diseases.